Protein purification: Fast forward

A fast and easy strategy for protein purification using “teabags”

Protein purification often involves affinity capture of proteins on stationary resin, alternatively proteins are captured on free flowing resin for subsequent separation from bulk fluid. Both methods require labour and time intensive separation of particulate matter from fluid. We present a method where affinity resin is contained within porous-walled containers, supporting clarification, produ...

Protein purification from whole tissues . Fatemeh Mehrpooyan*

EMBO Molecular Medicine: Fast Forward

These are exciting times for translational medicine as the convergence between fundamental and clinical research comes of age. The new EMBO Press publishing platform reinforces the standing of EMBO Molecular Medicine as the journal that matches high quality, novel research with rigorous editorial and ethical standards. It will also cement the journal's global reach and relevance whether in high...

Protein Purification

There are many thousand kinds of proteins with different properties and functions in a cell. To study a protein, one has to obtain the highly purified intact form of the protein. A major protein is not so difficult to be purified while a minor one may need many purification steps and high skills on the techniques. This chapter describes the principles underlying techniques for separation and pu...

Synthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification

Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...